Alteraciones histopatológicas en hámster inoculados con cepas leptospirales patógenas, recién aisladas por hemocultivos humanos
Keywords:
Hámsters, Histopatología, Cultivo de virusAbstract
Con el objetivo de demostrar alteraciones histológicas en diferentes órganos, fueron inoculados nueve hámsters de mas de 60 g. De masa por vía intraperitoneal, utilizando una carga infectiva de 0,5 ml de cepas virulentas de leptospiras. Se formaron cuatro grupos de tres animales cada uno, recibiendo cada grupo el inoculo con las cepas 4, 17, 22 y 43 pertenecientes a los serogrupos Ballum, Autumnalis y Australis respectivamente. Los animales fueron sacrificados a los tres meses de inoculados, procediéndose de inmediato a la extirpación de hígado, riñón, corazón y diafragma. Las muestras obtenidas de cada órgano fueron fijadas en formol al 10 % y procesadas siguiendo la técnica de H y E para su observación al microscopio de luz. En el examen histológico de los tejidos se encontraron como principales alteraciones, necrosis del hepatocito, infiltrado inflamatorio en el hígado y riñón y signos de nefritis.
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