Standardization of a model of colon cancer in BALB/c mice

Authors

  • María del Carmen Herrera de la Uz Facultad de Ciencias Médicas Dr Ernesto Che Guevara de la Serna
  • Yunit Hernández Rodríguez Facultad de Ciencias Médicas Dr Ernesto Che Guevara de la Serna
  • Isvet Rodríguez Peguero Facultad de Ciencias Médicas Dr Ernesto Che Guevara de la Serna
  • Mirian Teresita Guerra Paredes Facultad de Ciencias Médicas Dr Ernesto Che Guevara de la Serna
  • Agustín Lemus Sarracino Hospital General Docente "Abel Santamaría Cuadrado"

Keywords:

DeSC, Colon cancer / animal models / metastasis

Abstract

Introduction: colon cancer occupies the first place within the neoplasias of the digestive system and the second among all the onco-proliferative processes in both sexes. For the evaluation of vaccines and molecular mechanisms specific to the disease, murine models are frequently used, and there is no animal model standardized for these purposes in this context.

Objective: to standardize a RCC model by implanted cells, stable, reproducible and susceptible to be used in later studies.

Method: young female BALB / c mice were distributed in two control (n = 9) and one (n = 9) groups to which subcutaneous (ectopic) CT 26 cells were inoculated. After 35 days euthanasia was practiced and the morphological characteristics and the presence of spontaneous metastases in tumors were studied.

Results: in the animals of the study group, subcutaneous nodules with the characteristics of a low-grade colon adenocarcinoma were found, which were responsible for the weight gain observed in the study group, which was evidenced when the tumors were removed from the group. The weights of the organs studied were increased by tumor influences, but only spontaneous metastases were found in the lungs.

Conclusions: with this study it was possible to standardize a stable, reproducible and susceptible RCC model per implanted cell, which could be used in subsequent studies.

 


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Author Biographies

María del Carmen Herrera de la Uz, Facultad de Ciencias Médicas Dr Ernesto Che Guevara de la Serna

Especialista de primer grado en Fisiología normal y patológica. Instructor

Yunit Hernández Rodríguez, Facultad de Ciencias Médicas Dr Ernesto Che Guevara de la Serna

Especialista de segundo grado en Fisiología normal y patológica, Máster, profesor auxiliar, Investigador Agregado.

Isvet Rodríguez Peguero, Facultad de Ciencias Médicas Dr Ernesto Che Guevara de la Serna

Especialista de primer grado en Fisiología normal y patológica. Instructor

Mirian Teresita Guerra Paredes, Facultad de Ciencias Médicas Dr Ernesto Che Guevara de la Serna

Especialista de segundo grado en Fisiología normal y patológica, Máster, profesor auxiliar

Agustín Lemus Sarracino, Hospital General Docente "Abel Santamaría Cuadrado"

Especialista Segundo Grado en Anatomía Patológica, Prof. Auxiliar, Máster en Medios Diagnósticos Departamento de Anatomía Patológica

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Published

2016-12-23

How to Cite

1.
Herrera de la Uz M del C, Hernández Rodríguez Y, Rodríguez Peguero I, Guerra Paredes MT, Lemus Sarracino A. Standardization of a model of colon cancer in BALB/c mice. Rev Ciencias Médicas [Internet]. 2016 Dec. 23 [cited 2025 Aug. 1];20(6):671-82. Available from: https://revcmpinar.sld.cu/index.php/publicaciones/article/view/2660

Issue

Vol. 20 No. 6 (2016)

Section

ORIGINAL ARTICLES

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