Histological alterations due to hypervitaminosis A in the long bones of newly born Sprague Dawley rats

Authors

  • Rayza Hernández Díaz Facultad de ciencias Medicas de Pinar del Rio
  • José Guillermo Sanabria Negrín Universidad de Ciencias Médicas de Pinar del Río
  • Isvel Zaldívar Garrit Universidad de Ciencias Médicas de Pinar del Río.
  • Margarita Llanuch Lara Universidad de Ciencias Médicas de Pinar del Río.
  • Dunia Teresita Paredes lazo Universidad de Ciencias Médicas de Pinar del Río

Keywords:

VITAMIN A, HYPERVITAMINOSIS A, RATS, BONES.

Abstract

Introduction: the consumption in high doses of vitamin-A at a minimum teratogenic dose could be a possible cause of histological alterations of bones during intrauterine growth.

Objective: to assess the effect of hypervitaminosis A induced in rats on the long bones of offspring.

Method: an experimental study was conducted on pregnant Sprague Dawley rats: two groups with three animals each: negative control and cases with VA dose 50 μg / g during the first 16 days of pregnancy between September 2015 and February 2016. The right femurs of all rats were taken (n = 12 in each group) 2 hours after birth. The bones were decalcified, processed in paraffin and stained with haematoxylin / eosin. Longitudinal section morphometry of the right femur was performed at the midpoint of the diaphysis of the thickness of the periosteum, endosteum, cortical and total of the diaphysis. The qualitative variables were summarized by frequencies and the quantitative using the mean and standard deviation. The comparisons were verified by X2 and Student's-T tests at 95 % certainty.

Results: thickness of the periosteum and endosteum, cortex thickness, and diaphysis of the femur showed highly significant decreases (p <0.0001) when comparing the means between the study and control groups. Increased endochondral cartilage and dilation of medullar vessels were more frequent among cases (p <0.0001)

Conclusions: hypervitaminosis A during pregnancy in Sprague Dawley rats reduces the size of the femur in offspring.

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Author Biographies

Rayza Hernández Díaz, Facultad de ciencias Medicas de Pinar del Rio

Espècialista en Médicina General Integral, y Especialista de Primer Grado en Histología.  Profesora Asistente.

José Guillermo Sanabria Negrín, Universidad de Ciencias Médicas de Pinar del Río

Doctor en Medicina. Especialista de Segundo Grado en Histología. Dr. en Ciencias Biológicas. Profesor Consultante.

Isvel Zaldívar Garrit, Universidad de Ciencias Médicas de Pinar del Río.

Doctor en Medicina. Especialista de Primer Grado en Embriología. Profesor Asistente.

Margarita Llanuch Lara, Universidad de Ciencias Médicas de Pinar del Río.

Doctora en Medicina. Especialista de Segundo Grado en Histología. Profesora Auxiliar.

Dunia Teresita Paredes lazo, Universidad de Ciencias Médicas de Pinar del Río

Especialista 1er Grado en Histología. Profesor Instructor.

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Published

2018-03-02

How to Cite

1.
Hernández Díaz R, Sanabria Negrín JG, Zaldívar Garrit I, Llanuch Lara M, Paredes lazo DT. Histological alterations due to hypervitaminosis A in the long bones of newly born Sprague Dawley rats. Rev Ciencias Médicas [Internet]. 2018 Mar. 2 [cited 2025 Aug. 30];22(2):208-15. Available from: https://revcmpinar.sld.cu/index.php/publicaciones/article/view/3411

Issue

Vol. 22 No. 2 (2018)

Section

ORIGINAL ARTICLES

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