Hypervitaminosis A and hepatic tissue lesions in newly born Sprague Dawley rats
Keywords:
HYPERVITAMINOSIS A, RATS, LIVER DISEASES.Abstract
Introduction: hypervitaminosis A is capable of causing morpho-histological changes in different organs and tissues that can cause serious consequences in pregnant women and the product of their conception.
Objective: to evaluate the effects of hypervitaminosis A during pregnancy on liver tissue in broods of Sprague Dawley rats.
Method: an experimental study was carried out using Sprague Dawley rats bred in two groups, with two rats each one, between September 2015 and February 2016. Control group: no treatment. Experimental group: was given vitamin A, 50 μg / g during the first 16 days of pregnancy; 2 hours after the litter was born (n = 12 per group) the livers were analyzed by histology and morphometry. Qualitative and quantitative variables were studied. The first ones were compared by the X2 test; the quantitative ones through a test of means difference. All were verified at 95% of certainty.
Results: in the treated group a significant increase (p <0.05) of vacuolar hepatocyte degeneration, hyperplasia of the Küpffer cells and sinusoidal congestion were found. The hepatocellular necrosis and the congestion of the central veins were not different. A significant increase (p <0.05) in nuclear volume was found in the 3 zones of the hepatic acinus.Conclusions: hypervitaminosis A (50μg / g), during pregnancy in rats, produces liver alterations in newborns, therefore a balance in the intake of vitamin A, especially during pregnancy, it is necessary to contribute to a better quality of pregnant women, in order to improve Maternal and Child Program of the country.
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