Effect of vitamin E on endogenous antioxidants in diabetic Wistar rats
Keywords:
DIABETES MELLITUS, VITAMIN E, ALBUMINS, URIC ACID, BILIRUBIN.Abstract
Introduction: Diabetes Mellitus is one of the most common chronic noncommunicable diseases in the world, the fourth or fifth cause of death in most high-income countries. The World Health Organization reported in 2014 a prevalence of 422 million diabetics worldwide.
Objective: to assess the effect of vitamin E on endogenous nucleophilic antioxidants in a biomodel of streptozotocin-induced diabetes mellitus.
Methods: 40 male Wistar rats were used in four groups of 10 rats each: non-diabetic control, diabetic control, and two diabetic groups that received supplementation with doses of 25 and 50 mg / kg / day of vitamin E, respectively. Serum values of glucose, albumin, uric acid and total bilirubin were determined in all animals at 15-day intervals for one month. The U-tests of Mann-Whitney and Wilcoxon with a significance level of 5% were used to compare the central values of the biochemical variables between the groups of rats.
Results: with vitamin E supplementation in diabetic rats, no variation of serum albumin was found (median in g / L 36,70 and 36,40), while uric acid (median in mmol / L 76, 50 and 187.5) and BT (median in mmol / L 2.90 and 5.00) decreased significantly, regardless of the antioxidant dose. The reduction of uric acid was faster and at a lower dose than BT.
Conclusions: supplementation with vitamin E in the experimental model of diabetes mellitus, reduces serum levels of nucleophilic antioxidants, which at high concentrations represent a risk of morbid processes associated with tissue damage.
Downloads
References
Storino MA., Miguel A. Contreras, Jairo Rojano, Richard Serrano, Ana Nouel. Complicaciones de la diabetes y su asociación con el estrés oxidativo: un viaje hacia el daño endotelial. Revista Colombiana de Cardiología[Internet]. 2014 nov[citado 2016 Nov 15]; 21(6): 392-398. Disponible en: http://www.sciencedirect.com/science/article/pii/S0120563314000801
.
2 Eshaq RS, Wright WS, Harris NR. Oxygen delivery, consumption, and conversion to reactive oxygen species in experimental models of diabetic retinopathy. Redox Biol. [Internet]. 2014 Apr. 18[citado 2016 Nov 15]; 2: 661-666. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052533/
3 Okeoghene Ogbera A, Ezeobi E, Unachukwu C, Oshinaike O. Treatment of Diabetes Mellitus-associated neuropathy with vitamin E and Eve primrose. Indian J Endocr Metab 2014[citado 2016 Nov 15];18(6):846-849. Disponible en: http://www.ijem.in/article.asp?issn=2230-8210;year=2014;volume=18;issue=6;spage=846;epage=849;aulast=Ogbera
4 Fabbrini E, Serafini M, Colic Baric I, Hazen SL, Klein S. Diabetes. Effect of plasma uric acid on antioxidant capacity, oxidative stress, and insulin sensitivity in obese subjects Journal list Diabetes [internet]. 2014 Mar [citado 2016 Nov 16]; 63(3):976-981. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931399/
5 Heredia RD, Fernández CD, Alfonso RJ, Rodríguez VE, Santana GL, Rodríguez PM. Sistema antioxidante enzimático e indicadores de daño oxidativo en pacientes diabéticos tipo 2. Rev. chil. endocrinol. diabetes 2014[citado 2016 Nov 15]; 7 (3): 94-98. Disponible en http://www.revistasoched.cl/3_2014/4-heredia.html
6 Cumming KT, Raastad T, Holden G, Bastani NE, Schneeberger D, Paronetto MP. Effects of vitamin C and E supplementation on endogenous antioxidant systems and heat shock proteins in response to endurance training. Physiological Reports [internet]. 2014 [citado 2016 nov 15];2(10): e12142. Disponible en: https://physoc.onlinelibrary.wiley.com/doi/pdf/10.14814/phy2.12142
7 Obregón O, Vecchionacce H, Brito S, Lares M, Castro J, Ramírez X. Efecto antiglicosilante de las vitaminas E y C. AVFT [internet]. 2005 [citado 2016 nov 15];24(1):1-7. Disponible en: http://www.redalyc.org/articulo.oa?id=55924110
8 Rodiño Janeiro BK, Raposeiras Roubin S, González Peteiro M, Ucieda Somoza F, González-Juanatey JR, Álvarez Castro E. La Albúmina Glicada Aumenta la Producción de Especies Reactivas del Oxígeno en el Endotelio Mediante la Activación del NF-kβ y el Desacoplamiento de la Sintasa Óxido Nítrico Endotelial. Rev Esp Cardiol [internet]. 2011 [citado 2016 nov 15];64(Supl.3):157. Disponible en: http://www.revespcardiol.org/controladores/congresos-herramientas.php?idCongreso=2&idSesion=276&idComunicacion=3091
9 Mendoza Coussette U, García Piñeiro JC, Gastell PL, Amador Armenteros A. Xantina oxidorreductasa, propiedades, funciones y regulación de su expresión genética. Rev Cubana Invest Bioméd [internet]. 2005 [citado 2016 nov 15];24(2). Disponible en: www.scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-03002005000200007
10 López Gómez L. Consenso Venezolano sobre Ácido Úrico Como Factor de Riesgo Cardiovascular [internet]. 2016 [citado 2016 nov 15]: 1-130. Disponible en: http://www.svemonline.org/wp-content/uploads/2016/04/consenso-venezolano-sobre-acido-urico.pdf.
11 Sierra Ortega YG, Rodríguez González G. Diabetes Mellitus y estrés Oxidativo 2. Hospital Princess Margaret. Terapia Intensiva [internet]Revista Médica Electrónica. 2014 [citado 2016 nov 15]. Disponible en: http://www.revista-portalesmedicos.com/revista-medica/diabetes-mellitus-estres-oxidativo/
12 Sánchez M, Maribel C, Rodríguez A, Rubén A, Martín V, Sepúlveda P. Estrés y vitaminas antioxidantes en pacientes diabéticos Tipo 2. Archivos Venezolanos de Farmacología y Terapéutica [internet]. 2008 [citado 2016 nov 15];27(1): 59-65. Disponible en: http://www.redalyc.org/articulo.oa?id=55927110
13 Jansen E, Viezeliene D, Beekhof P, Gremmer E, Ivanov L.Tissue-Specific Effects of Vitamin E Supplementation Int. J. Mol Sci[internet] .2016[citado 2016 nov 25];17(7): 1166; doi:10.3390/ijms17071166. Disponible en: http://www.mdpi.com/1422-0067/17/7/1166/htm
Published
How to Cite
Issue
Section
License
Authors who have publications with this journal agree to the following terms: Authors will retain their copyrights and grant the journal the right of first publication of their work, which will be publication of their work, which will be simultaneously subject to the Creative Commons Attribution License (CC-BY-NC 4.0) that allows third parties to share the work as long as its author and first publication in this journal are indicated.
Authors may adopt other non-exclusive license agreements for distribution of the published version of the work (e.g.: deposit it in an institutional telematic archive or publish it in a volume). Likewise, and according to the recommendations of the Medical Sciences Editorial (ECIMED), authors must declare in each article their contribution according to the CRediT taxonomy (contributor roles). This taxonomy includes 14 roles, which can be used to represent the tasks typically performed by contributors in scientific academic production. It should be consulted in monograph) whenever initial publication in this journal is indicated. Authors are allowed and encouraged to disseminate their work through the Internet (e.g., in institutional telematic archives or on their web page) before and during the submission process, which may produce interesting exchanges and increase citations of the published work. (See The effect of open access). https://casrai.org/credit/
