Morphometric changes produced on the testicular parenchyma in Wistar rats treated with bee honey

Authors

  • Yadira Lobo Romero Universidad de Ciencias Médicas de Pinar del Río. Facultad de Ciencias Médicas "Dr. Ernesto Che Guevara de la Serna" https://orcid.org/0000-0002-7525-3059
  • Isvel Zaldivar Garit Universidad de Ciencias Médicas de Pinar del Río. Facultad de Ciencias Médicas "Dr. Ernesto Che Guevara de la Serna" https://orcid.org/0000-0002-9199-501X
  • Marvelia Díaz Calzada Universidad de Ciencias Médicas de Pinar del Río. Policlínico Universitario “Luis Augusto Turcios Lima”. Pinar del Río, Cuba. https://orcid.org/0000-0002-0148-5914
  • Raiza Hernández Díaz Universidad de Ciencias Médicas de Pinar del Río. Facultad de Ciencias Médicas “Dr. Ernesto Che Guevara de la Serna”. Pinar del Río, Cuba https://orcid.org/0000-0003-4887-763X
  • Dianavell Morejon González Universidad de Ciencias Médicas de Pinar del Río. Facultad de Ciencias Médicas “Dr. Ernesto Che Guevara de la Serna”. Pinar del Río, Cuba https://orcid.org/0000-0001-7588-606X
  • Amado Crespo Dueñas Universidad de Ciencias Médicas de Pinar del Río. Facultad de Ciencias Médicas “Dr. Ernesto Che Guevara de la Serna”. Pinar del Río, Cuba https://orcid.org/0000-0002-7528-7099

Keywords:

INFERTILITY, MALE, HONEY, RATS, WISTAR.

Abstract

Introduction: Infertility is becoming more and more common in developed societies. It is estimated that there are 60-80 million infertile couples worldwide and at least 30 million men suffer from infertility.

Objective: to describe the morphometric changes of the testicular parenchyma in Wistar rats treated with bee honey administration.

Methods: a non-observational, basic, experimental, standard clinical trial type study was carried out in Wistar rats at the University of Medical Sciences of Pinar del Río, in the period September 2019-September 2021. The sample consisted of 27 specimens, which were distributed in three groups: group 1 (1g/miel/kg), group 2 (3g/miel/kg) and group 3 (control).  The extracted testes were studied with the elaboration of histological slides.

Results: There was a statistically significant variation in the diameter, area and volume of the nucleus and perimeter of Leydig cells (p<0.001) between groups, where the highest values were found in group 2. A greater smaller diameter (0,72 ± 0,12) and a greater larger diameter (0,92 ± 0,10) were found in group 2, with statistically significant inter-group variations (p<0,001).

Conclusions: in male Wistar rats, the administration of bee honey produces a dose-dependent increase in the diameter, area, perimeter and volume of the nucleus of Leydig cells, as well as in the greater and smaller diameters of the seminiferous tubules.

Downloads

Download data is not yet available.

References

1. Salder TW. Langman. Embriología médica. 2019. editorial Wolters Kluwer.

2. Quintero Vásquez GA, Bermúdez Cruz RM, Castillo Cadena J. Infertilidad masculina y fragmentación del ADN espermático: un problema actual. Rev Especializada en Ciencias Químico-Biológicas [Internet]. 2015 Dic [citado 24/11/2021]; 18(2): 144-51. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-888X2015000200144

3. Nicot Vidal LF, Quevedo Durand A, Nicot Crespo Y. Dinámica e impacto de la atención a parejas con infertilidad. Hospital Provincial Guantanamo 2011-2014. Rev Inf Cient [Internet]. 2016 [citado 24/11/2021]; 95(5): 711-20. Disponible en: https://dialnet.unirioja.es/servlet/articulo?codigo=6027478

4. Rodríguez Fernández MC. Modelo vincular en una pareja tratada a causa de infertilidad. MEDISAN [Internet]. 2016 Oct [citado 24/11/2021]; 20(10): 2294-7. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1029-30192016001000014&lng=es

5. Salvatierra Maza PL, Villegas Gómez LF. Alteraciones más frecuentes de los parámetros seminales en muestras de pacientes, Laboratorio biogénesis [Tesis]. Lima, Perú: Universidad privada Norbert Wiener [Internet]; 2016 [citado 24/11/2021]. Disponible en: http://repositorio.uwiener.edu.pe/handle/123456789/1394

6. Uribe Muñante MF. Estudio comparativo sobre la calidad seminal entre la población de jóvenes y adultos en el Departamento de Lima – Perú [Tesis]. Lima, Perú: Universidad Ricardo Palma [Internet]; 2017 [citado 08/12/2021]. Disponible en: https://repositorio.urp.edu.pe/handle/20.500.14138/1712

7. Lafuente Varea RS. Efecto de los factores ambientales en la calidad espermática del hombre [Tesis]. Barcelona, España: Universidad Autónoma de Barcelona [Internet]; 2017 [citado 24/11/2021]. Disponible en: https://ddd.uab.cat/pub/tesis/2017/hdl_10803_456559/rslv1de1.pdf

8. Ramírez Moran AF, Cala Bayeux A, Fajardo Iglesia D, Grave de Peralta RS. Factores causales de infertilidad. Rev Información Científica [Internet]. 2019 [citado 24/11/2021]; 98(2): 289-7. Disponible en: http://www.revinfcientifica.sld.cu/index.php/ric/article/view/2235

9. Pacheco L, Lozano Hernández R, Velazco J. Agentes infecciosos infrecuentes que afectan los parámetros seminales y la fertilidad. Rev Cubana Urol [Internet]. 2020 [citado 24/11/2021]; 9(3):128-42. Disponible en: http://www.revurologia.sld.cu/index.php/rcu/article/view/592

10. Gómez Guardo M. Esterilidad e infertilidad: El problema que invade las consultas [Tesis]. España: Universidad de Cantabria [Internet]; 2016 [citado 08/12/2021]. Disponible en: https://repositorio.unican.es/xmlui/bitstream/handle/10902/9560/Gomez%20Guardo%20Maria.pdf?sequence=4

11. Griggs G, Bird J. Quick facts about infertility [Internet]. USA: American Society for Reproductive Medicine; 2017 [citado 24/11/2021]. Disponible en: https://www.reproductivefacts.org /faqs/quick-facts-about-infertility/

12. Practice Committee of the American Society for Reproductive Medicine. Report on varicocele and infertility. Fertil Steril [Internet]. 2014 Dec [citado 24/11/2021]; 102(6): 1556-60. Disponible en: https://doi.org/10.1016/j.fertnstert.2014.10.007

13. Pardo Gambarte L. El testículo: estructura, función y patología testicular más frecuente. [Tesis]. Santander, España: Universidad de Cantabria [Internet]. 2017 Junio [citado 24/11/2021]. Disponible en: https://repositorio.unican.es/xmlui/bitstream/handle/10902/11694/Pardo%20Gambarte%20Laura.pdf?sequence=4

14. Roldán Rodríguez AE, Vega Quispe EJ. Efecto gastroprotector de la miel de abeja en ratas Holtzman con úlceras. Rev Gastroenterol Peru [Internet]. 2016 [citado 08/12/2021]; 36(3):. Disponible en: http://www.scielo.org.pe/scielo.php?pid=S1022-51292016000300005&script=sci_abstract

15. Hassan S, Zahran F, Watad S. Homing of HSCS From their niche by Honey bovine Colostrum for treatment of induced infertility in male mice. GSJ [Internet] 2019 Feb[ citado 08/12/2021];7(2) . Disponible en: https://www.globalscientificjournal.com/researchpaper/Homing_of_HSCs_From_Their_Niche_by_Honey_And_Bovine_Colostrum_For_Treatment_Of_Induced_Infertility_In_Male_Mice.pdf

16. Sánchez Soto R. Evaluación de la Miel de Abeja como Diluyente en la Conservación del Semen Porcino [Internet]. México: Tecnológico Nacional de México; 2020 [citado 08/12/2021]. Disponible en: https://rinacional.tecnm.mx/handle/TecNM/1116

17. Díaz EG, Calleja NP, Valle RP, Leyva ER, Abreu AF. La superación en Fitoterapia y Apiterapia: Su importancia social como herramientas terapéuticas en la Atención Primaria de Salud. MEDICIEGO [Internet]. 2014 [citado 08/12/2021]; 20(2): 1-12. Disponible en: https://revmediciego.sld.cu/index.php/mediciego/article/view/142

18. OPS/OMS-Cuba. Medicina Natural y Tradicional [Internet]. Washington: Organización Panamericana de la Salud; 2017 [citado 08/12/2021]. Disponible en: https://www3.paho.org/cub/index.php?option=com_content&view=article&id=289:medicina-natural-tradicional&Itemid=282

19. Syazana NS, Hashida NH, Majid AM, Durriyyah Sharifah HA, Kamaruddin MY. Effects of Gelam Honey on Sperm Quality and Testis of Rat. Sains Malaysiana [Internet]. 2011[citado 08/12/2021]; 40(11): 1243–6.Disponible en: https://core.ac.uk/download/pdf/11491804.pdf

20. Vega Badillo J. Alteraciones en la homeostasis del colesterol hepático y sus implicaciones en la esteatohepatitis no alcohólica. TIP Rev Esp Cienc Quím Biol [Internet]. 2017 [citado 08/12/2021]; 20(1): 50-65. Disponible en: https://www.medigraphic.com/pdfs/revespciequibio/cqb-2017/cqb171e.pdf

21. Hassan Hadi I. Effect of Honey on Sperm Characteristics and pregnancy rate in mice.

Bull. Iraq nat. Hist. Mus [Internet]. 2017 [citado 08/12/2021]; 14(3) 2017: 223-33. Disponible en: https://www.iasj.net/iasj/download/1531d8e28b9079fd

22. Ruiz P, Genovese P, Bielli A, Herrera E, Pautassi RM. La Reserpina Aumenta la Expresión de BDNF y PCNA, y Disminuye la de Caspasa-3, en Células Intersticiales (Células de Leydig) de Ratas. Int J Morphol [Internet]. 2018 Sep [citado 08/12/2021]; 36(3): 895-900. Disponible en: https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000300895&lng=es

Downloads

Published

2022-12-16

How to Cite

1.
Lobo Romero Y, Zaldivar Garit I, Díaz Calzada M, Hernández Díaz R, Morejon González D, Crespo Dueñas A. Morphometric changes produced on the testicular parenchyma in Wistar rats treated with bee honey. Rev Ciencias Médicas [Internet]. 2022 Dec. 16 [cited 2025 Aug. 22];26(6):e5630. Available from: https://revcmpinar.sld.cu/index.php/publicaciones/article/view/5630

Issue

Vol. 26 No. 6 (2022)

Section

ORIGINAL ARTICLES

License

Authors who have publications with this journal agree to the following terms: Authors will retain their copyrights and grant the journal the right of first publication of their work, which will be publication of their work, which will be simultaneously subject to the Creative Commons Attribution License (CC-BY-NC 4.0) that allows third parties to share the work as long as its author and first publication in this journal are indicated.

Authors may adopt other non-exclusive license agreements for distribution of the published version of the work (e.g.: deposit it in an institutional telematic archive or publish it in a volume). Likewise, and according to the recommendations of the Medical Sciences Editorial (ECIMED), authors must declare in each article their contribution according to the CRediT taxonomy (contributor roles). This taxonomy includes 14 roles, which can be used to represent the tasks typically performed by contributors in scientific academic production. It should be consulted in monograph) whenever initial publication in this journal is indicated. Authors are allowed and encouraged to disseminate their work through the Internet (e.g., in institutional telematic archives or on their web page) before and during the submission process, which may produce interesting exchanges and increase citations of the published work. (See The effect of open access). https://casrai.org/credit/